Purification:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
Recombinant:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
MTT Assay:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
Enzyme-linked Immunosorbent Assay:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
Reverse Transcription:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
Multiplexing:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
Cytokine Assay:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
Software:Article Title: Anti-SARS-CoV-2 Spike IgA2 Induces Inflammation by Human Macrophages.
Article Snippet: .. Reagent Source Identifier Antibodies Anti-CD89, MIP8α LifeSpan BioSciences LS-C188187 Mouse-anti-human IgA1 clone MH141-1 Sanquin In-house production Mouse-anti-human IgA2 clone A9604D2 Southern Biotech Cat. 9140-05 Purified human pooled serum IgA (psIgA) MP Biomedicals SKU: 0855906 Chemicals, peptides, and recombinant proteins 2-Deoxy-D-glucose MedChemExpress HY-13966 3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) Sigma-Aldrich 475989 6-Aminonicotinamide MedChemExpress HY-W010342 Alpelisib Selleck Chemicals S2814 C75 MedChemExpress HY-12364 Duvelisib MedChemExpress HY-17044 Entospletinib (GS-9973) Selleck Chemicals Cat. S7523 Etomoxir Sigma-Aldrich 236020 Human GM-CSF Miltenyi Biotec Cat. 130-093-864 Human M-CSF Miltenyi Biotec Cat. 130-096-491 Idelalisib MedChemExpress HY-13026 L-lactate Sigma-Aldrich L7022-5G Polyinosinic:polycytidylic acid (Poly(I:C)) Sigma-Aldrich Cat. P1530 Recombinant human IL-10 protein R&D Systems Cat. 217-IL-025/CF SARS-CoV-2 (Wuhan-Hu-1) spike Sanquin https://doi.org/10.1126/science.abc5902 Critical commercial assays CD14 MicroBeads, human Miltenyi Biotec Cat. 130-050-201 ELISA MAX Standard Set Human IL-6 BioLegend Cat. 430501 High-capacity cDNA reverse transcription kit ThermoFisher Scientific 4368814 Multiplexing human cytokine assay kit U-PLEX Meso scale discovery, inc. K15049D, K15047D RNeasy mini kit Qiagen Cat. 74104 SensiFAST SYBR No-ROX Kit Bioline BIO-98005-BL Software Discovery Workbench Software version 4.0 Meso scale discovery, inc. www.mesoscale.com GraphPad Prism version 10.2.0 GraphPad Software www.graphpad.com 12 of 15 European Journal of Immunology, 2025 nloaded from https://onlinelibrary.w iley.com /doi/10.1002/eji.70068 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [10/10/2025]. ..
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